Radical scavenging activity of plant extracts from improved processing. As positive controls, epicatechin and lascorbic acid were also examined for dpph radical scavenging activity. On the other hand, the above mentioned standard antioxidants indicated an inhibition of 92. However, there has been no report on the antioxidant properties of this species.
The antioxidant activity increased with an increase in concentration of the extracts reaching a plateau. Free radical scavenging activity and antioxidants of. Salinity is a widespread environmental stress that severely limits crop yield worldwide. Radical scavenging potential and cytotoxic activity of phenolic compounds from tectona grandis linn. Conversely, the essential oil of anise in which the percentage of monoterpenes was as low as 2. The objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s. Antioxidant and free radical scavenging potential have been reported to. Dpph free radical scavenging assay the free radical scavenging activity of the extract was measured using dpph by the method of blios 1958. Nitric oxide scavenging activity, antioxidant activity, active nitrogen species. Screening of in vitro antioxidant activity of methanolic. Free radical scavenging activity of seed of holarrhena. The extract was examined for different reactive oxygen species ros scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and. Ataturk university, faculty of sciences, department of chemistry, 25240erzurum, turkey. Abutilon indicum, total phenolic content tpc, antioxidant activity, oxidative stress, dpph scavenging activity.
Toxic, radical scavenging, and antifungal activity of. Evaluation of antioxidant and free radical scavenging. Oct 30, 2015 the free radical scavenging activity of different extracts and bht was in the following order. The weakest radical scavenging potential against dpph shows. Radical scavenging and antioxidant capacity of serotonin volume.
Ten methanol extracts from various parts of seven medicinal plants, angiopteris evecta hoffm, marattiaceae citrus hystrix dc, rutaceae laurentia. The ic 50 values table 1 of the extract and standard in this assay were 112. Antioxidant activity could be measured simply by placing the fat in a closed container with oxygen and measuring the rate of oxygen consumption. Chrysin, rutin and quercetin were run to explore the effect of.
The antioxidant activity of the memq was evaluated by the. Leaves article pdf available january 2014 with 11,810 reads. In this study, free radical scavenging activity, total phenolic content, total oxidant level, and total antioxidant level of methanol and acetone extracts of t. Free radical scavenging activity of crude extracts and 4. Evaluation of nitrite radical scavenging properties of. Hydroxyl radical scavenging activity assay figure 4. The total phenolic content of the extracts was found to be higher in a. Box 67, dschang, cameroon 1 department of biochemistry. Isolation, synthesis, and radicalscavenging activity of. Reducing power assay 0 20 40 60 80 100 0 100 200 300 400 500 % concentration gml dpph radical scavanging activity ascorbic acid sida acuta 0 10 20 30 40 50 60 70 90 0 100 200. To measure the antioxidant activity of eps7630 the dpph. Antioxidant and free radical scavenging activity of spondias.
Thus it could be concluded that the aia showed a potent total phenolic content and possessed a significant scavenging activity. Means and standard deviations of the data were presented. Pdf radical scavenging potential and cytotoxic activity. Antioxidant and free radical scavenging potential of. The polyphenolic content and free radical scavenging activity of plants that had passed all stages of cultivation. Free radical scavenging activity screening of medicinal. Xanthine oxidase inhibitory and dpph radical scavenging. Hydrodynamic and tem measured diameter of synthesized melaninlike nanoparticles with different synthetic parameters figure s1. Free radical scavenging activity determination and quantitative analysis of curcuminoids in curcuma zedoaria rhizome extracts by hplc method. Phytochemicals are bioactive compounds found in vegetables, fruits, cereal grains, and plant. Evaluation of abelmoschus moschatus extracts for antioxidant. In the above assays, crude extract and all the fractions showed antioxidant potential to varying degrees. Among them, the 2,2diphenyl1picrylhydrazyl dpph spectrophotometric method is one of the most widely applied and is appreciated for its reliability.
Structures of chlorophylls a and b and pheophytins a and b. The antioxidant and free radical scavenging activity of the ethanol extracts of melia dubia hiern. The leaf and flower extracts inhibited lipid peroxidation induced by. Antioxidant capacity and radical scavenging effect of. It is the most important oxidant for carbon monoxide, methane and other hydrocarbons, sulfur dioxide, hydrogen sulfide, and most of other contaminants, removing them from the atmosphere. The differences between the free radical scavenging activity of laboratory and production. In addition, aafb melanin exhibited stronger scavenging activities on 2,2 diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. Screening of radical scavenging activity and polyphenol content of.
The antioxidant activity of the aerial part extract of m. Free radical scavenging activity and total flavonoid. Free radical scavenging activity was determined according to the elimination of dpph radicals and total phenol content. Dpph is a stable free radical in a methanolic solution. The antioxidant activity of the extracts was measured on the basis of the scavenging activity of the stable 1, 1 diphenyl 2picrylhyorazyl dpph free radical according to the method described by brandwilliams et al22 with slight modifications. In its oxidized form, the dpph radical has an absorbance maximum centered at about 520 nm molyneux, 2004. Free radicalscavenging capacity, antioxidant activity and. Ten 10 sample preparations were done for the radical scavenging activity with ten 10 different concentrations such as 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 gml. The method dpph is widely used for measurement of free radical scavenging ability of antioxidants perezjimenez and sauracalixto, 2008. The compound sodium nitroprusside is known to decompose in aqueous solution at physiological ph 7.
These results indicate that the high abts radical scavenging ability of each t. The dpph radical himedia is stable due to the delocalization of a spare electron over the molecule, thus preventing dimer formation. All the extracts showed different levels of dpph radical scavenging activity over the range of 210 mgml concentration and the ec50 value of aqkf, mkf, ackf, ckf and pekf was found to be 4. Among them, thyme and oregano exhibited the highest antioxidant activity, with i dpph values of 98. Pdf antioxidant activity by dpph radical scavenging method. Dpphfree radical scavenging capacity of legume extracts was evaluated according to the method of chen and ho 11 as modified by xu and chang 10. Serotonin, antioxidant activity, metal chelating, reducing power, radical scavenging. The ic50 value table 1 of the test sample was centage inhibition of deoxyribose degradation. Cerium oxide nanoparticles nanoceria have the unique capability of catalytically reducing levels of stressinduced reactive oxygen species ros including hydroxyl radicals. The maximum free radical scavenging activity and potency were interpolated from figure 1 to give results as shown in table 1. The ability to scavenge free radicals indicates these plants could be used as a new source of antioxidant agents, and the activity seen. In vitro antioxidant and free radical scavenging activity of. Hydroxyl radical scavenging by cerium oxide nanoparticles.
Antioxidant and free radical scavenging activity of certain. Nev ertheless, strong correlation was obtained be tween ph, color, electrical conductivity and total soluble solid with the scavenging activity of all honey samples with the correlative coefficient, r 0. To collect useful items by searching through refuse. For determination of radical scavenging activity of different foods, beverages and substrates were elaborated a. Percentage of dpph free radical scavenging activity was calculated as follows choi et al. The table 2, 3, and 4 shows the antioxidant activities of the ethanol, methanol and hexane extracts of six green leafy vegetables glvs assessed using the dpph radical scavenging. In the dpph free radical scavenging activity, three coumarin compounds i, ii and iii were evaluated for their free radical scavenging activity with ascorbic acid as standard compound. Many plant phenolic flavonoids have been shown to have antioxidant activity.
All the essential oils showed antioxidant activity. Determination of free radical scavenging activity of plant. Numerous attempts have been made to relate the free radical scavenging capacity of compounds to their antioxidant activity in foods even though antioxidant activity is dependent on both physical and chemical properties. Method principle 1,1diphenyl2picrylhydrazyl dpph is a free stable radical with purple colour. Antioxidative and free radical scavenging activities of some plants. Antioxidant and free radical scavenging activity of.
New acylated phenolic glycosides with ros scavenging. In our study, the polyphenolic content and the radical scavenging activity of a number of thai fruit extracts were determined by folinciocalteu reagent and scavenging of dpph 2,2diphenyl1picrylhydrazyl radical. Although puerarin is wellknown for its effective antioxidant activity, there is seldom a systematic theoretical study on its radical scavenging activity. Box 719, hr31107 osijek, croatia bthe rugjer bokovi institute, p. In atmospheric chemistry, the most common scavenger is the hydroxyl radical, a shortlived radical produced photolytically in the atmosphere.
In vitro antioxidant and free radical scavenging activity. The order of potency of scavenging hydroxyl radical was egcgfaga. Several methods have been developed to assess the radical scavenging activity. The order of potency of scavenging superoxide anion, nitric oxide no and dpph was egcgfacga. The scavenging activity of the plant extract through the annihilation of the dpph radicals was investigated. The hopping increases additionally the values of the parameter.
The antioxidant activities of different concentrations of drugs were determined by total polyphenolic content, ascorbic acid, total flavonoids, dpph radical. Invitro antioxidant and free radical scavenging activity of. Puerarin is a cglycoside of daidzein, one of the major bioactive ingredients isolated from the root of pueraria lobata, which has a wide spectrum of pharmacological effects. A 1 is the absorbance of dpph solution with test extract and a 2 is the absorbance of blank. Phytochemical screening and free radical scavenging activity. In vitro nitric oxide scavenging activity of methanol. Mechanism of the oh radical scavenging activity of. This paper presents data on the antioxidant and chela tion properties of chlorophyll a and b and pheophytin a.
Dpph radical scavenging activity of extracts from rhamnus. The measurement of the dpph radical scavenging activity was performed according to methodology described by rekha et al. The superoxide radical scavenging activity of seed and leaf extracts of a. Estimation of antioxidant activity a dpph radical scavenging activity. The samples were analysed by the antioxidant assay based on the scavenging of 1,1diphenyl2picrylhydrazyl dpph free radicals. A comparative study on the antioxidant activity of. Structureradical scavenging activity relationships of. Radical scavenging and antioxidant capacity of serotonin. Though the dpph stable free radicals are useful to assess the free radical scavenging activity, the concentration of dpph free radicals to be used is not a standard. Assay of nitric oxide scavenging activity nitric oxide scavenging activity can be estimated by the use of griess iliosvoy reaction garrat, 1964.
Free radical scavenging activity of ethanolic extract. Increasing the concentration of the extracts did not result in an increase in the nitrite radical scavenging activity. Radical scavenging and antioxidant activity of tannic acid. According to the data, glycosides of many phenolic hydroxyl groups lend a marked antioxidant activity to these molecules, and polyphenols 3mn and 3uv. Dpph radical scavenging activity of various leaf extracts of k.
Scavenging activity on dpph radical quantitative fig. Beyza ozturk sarikaya and ilhami gulcin affiliation. Oxygen radical scavenging activity of flavonoidschemistry, metabolism, cardioprotective effects, and dietary sources. All the plant extracts under study were found to be effective scavengers of dpph radicals along with considerable reducing ability. Dpph inhibition in mpe was determined by using the protocol of brandwilliams et al. Using dpph radical scavenging assay to measure antioxidants. Bioinspired polymerization of dopamine to generate melanin. Freeradical scavenging activity screening of some indonesian plants original article. Free radical scavenging activity, total phenolic content. In addition, aafb melanin exhibited stronger scavenging activities on 2,2diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. Dpph free radical scavenging activity of the extracts of the. M nitroblue tetrazolium nbt dissolved in 50 mm phosphate buffer ph 7.
Free radical scavenging activity, antioxidative ingredients, and. M 12 200 200 14 200 15 200 16 200 17 200 18 200 19 200 20 200 21 200 22 200 23 200 24 200 25 200 26 200 27 200 28 200 ascorbic acida 108. Free radical scavenging activity of plant extracts was evaluated using a 1,1diphenyl2picrylhydrazyl dpph assay. To search through a place or container for useful items. During the different stages of the brewing process the free radical scavenging activity is changed. The leaves extracts showed scavenging activity ranging from 03. Determination of dpph radicals scavenging activity was estimated with the method used by kato 5. In order to assess the radical scavenging potential of the crude extract, fractions i, ii and the compounds isolated, the reactivity towards the stable free radical dpph was measured at 518 nm by measurement of absorbance decrease of the reaction mixture after 5 hourreaction time. Antioxidants free fulltext radical scavenging activity. The free radical scavenging activity of the extract was measured in terms of hydrogen donating or radical scavenging ability using the stable free radical dpph 6, 7. Free radical scavenging activity of crude extracts and 4 bioline. Free radical scavenging activity and lipoxygenase inhibition. Free radicals, formed in the course of normal cellular respiration and metabolism, and more abundantly under the influence of certain environmental chemicals and sunlight, have been inculpated in various types of tissue damage, particularly those involved in atherosclerosis, the aging process, and the development of cancers.
Percentage of dpph free radical scavenging activity % where a 0 is the absorbance of negative control. Pdf free radical scavenging activity determination and. Radical scavenging activity and inhibition of macrophage. Aug 17, 2011 superoxide radical scavenging activity. Comparatively, methanolic extract showed higher activity than ethanolic activity. Scavenging definition of scavenging by the free dictionary.
The objective of this study was to compare the free radical scavenging activity of various compounds to their ability to inhibit lipid oxidation in foods. Total antioxidant activity, metal chelation, radical scavenging dpph effects and reducing power as well as activities destructive to active oxygen species such as the superoxide anion radical, hydroxyl radical, and hydrogen peroxide are widely used for this purpose shimada et al. In vitro antioxidant activity of coumarin compounds by dpph. Dpph free radical scavenging activity of compounds 12. Dpph radical scavenging capacity of phenolic extracts from. Hydroxyl radical scavenging activity the hydroxyl radical scavenging activity was determined according to the method of beara et al. Evaluation of free radical scavenging activity of various.
Comparative study of abts radical scavenging activity and. The dpph method is described as a simple, rapid and convenient method independent of sample polarity for screening of many samples for radical scavenging activity koleva et al. Phytochemical and free radical scavenging activity of poorna. Dpph radical scavenging assay dpph radical scavenging activity was measured as per standard method using stable dpph radical7. The establishment of the statistical correlation could. Evaluation of antioxidant and free radical scavenging activity of annona muricata santhoshkumar muthu and brindha durairaj department of biochemistry, psg college of arts and science, coimbatore. Free radical scavengers definition of free radical. Herein, the free radical scavenging ability of puerarin was. Dpph radical scavenging activity is one of the most widely used method for screening the antioxidant activity of plant extract. Synthesis of salidroside analogues and their ability of. Oh that lack enzymatic scavenging pathways nanobio interactions best papers 2018 environmental science. Phytochemical analysis, antimicrobial and radicalscavenging. However, it was the identification of vitamins c and e as antioxidants that revolutionized the field and led to the realization of the importance of antioxidants in the biochemistry of living organisms.
Bioinspired polymerization of dopamine to generate melaninlike nanoparticles having an excellent free radicalscavenging property kukyoun ju, yuwon lee, sanghee lee, seung bum park and jinkyu lee table s1. The antioxidant activity was also calculated as free radical scavenging activity % 1 a 1 a o100 where a 1 absorbance of sample at 517 nm a 0 absorbance of control at 517 nm data analysis samples were analysed in triplicate. Fa inhibited the lipopolysaccharidestimulated no production and inducible no synthase protein and mrna expression by mouse macrophagelike cells raw 264. One of the quick methods to evaluate antioxidant activity is the scavenging activity on dpph, a stable free radical and widely used index. Chemical composition and radical scavenging activity of. A comparative study on the antioxidant activity of methanolic. The dpph free radical scavenging activity was calculated using the following formula. The oil obtained from shoots gathered in september during the seedripening stage and containing appreciable amounts of palustrol 26. Relationships between free radical scavenging and antioxidant. In this study, free radical scavenging activity, total phenolic content, total oxidant status tos, and total antioxidant status tas of methanol ttm and acetone tta extracts of t. The crude aqueous extract of the plant contains the phenolics and flavonoids which are said to be the potent antioxidants 11.
Hydroxyl radical scavenging activity of the extracts was determined according to the method reported by klein et al. Antiradical scavenging activity was tested by the dpph model table 5. Extraction and determination of antioxidant activity of. In vitro antioxidant and free radical scavenging activity 39 roots are the main portions of the whole plant as they possess wide number of the therapeutic agents. Hydroxyl radical scavenging activity hrsa of the extractives was determined by the method of klein et al. Dpph free radical scavenging activity of the extracts of. Box 180, hr2 zagreb, croatia received march 26, 2002.
Structure radical scavenging activity relationships of flavonoids dragan ami,a, duanka davidoviami,a drago belo,a and nenad trinajstib afaculty of agriculture, the josip juraj strossmayer university, p. One of them is family lamiaceae which has spearmint plant. When dpph is mixed with a substrate that can donate a hydrogen atom antioxidants, then this gives rise to the reduced form with the loss of this violet color. Antioxidant activity by dpph radical scavenging method of ageratum conyzoides linn. Pdf antioxidant activity by dpph radical scavenging. The antioxidant and free radical scavenging activities of. Free radical scavenging activities of solutions of the plant extracts and synthetic antioxidant substances used in the study prepared in methanol at concentrations of 50, 100 and 200. Comparative study of in vitro, ex vitro and in vivo grown. Superoxide dismutase activity, oh radical scavenging and reducing power assay. Water and ethanol extracts of roots from cassine transvaalensis burttdavy. This paper presents data on the antioxidant and chela. Physicochemical and radical scavenging activities of honey. Structureradical scavenging activity relationships of flavonoids.
1292 1239 981 1536 1188 436 254 1356 662 929 456 1092 524 221 521 1075 1026 1328 723 1387 1509 1259 1095 68 23 374 219 1359 862 817 1216 1504 862 1065 313 930 977 644 1356 1336 1427 269 342 553 1249 485 1328 908 19